Composite

Part:BBa_K2983173:Design

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-15)


hrGFP expression cassette (BBa_K2983073) in YL-pOdd1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1727
    Illegal PstI site found at 1692
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2744
    Illegal SpeI site found at 1727
    Illegal PstI site found at 1692
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
    Illegal XhoI site found at 1281
    Illegal XhoI site found at 1314
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1727
    Illegal PstI site found at 1692
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1727
    Illegal PstI site found at 1692
    Illegal NgoMIV site found at 1126
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1520
    Illegal SapI.rc site found at 2752


Design Notes

BBa_K2983073 in BBa_K2983030


Source

DNA synthesis and assembly

References